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The quality of the BLUelf Prestained Protein Ladder is tested on a lot-to-lot basis to ensure consistent product quality. Sizing of proteins on SDS-PAGE and Western blots.Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25☌), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol. experiment by running Criterion TGX gels with five prestained protein standards (Thermo Fisher SeeBlue Plus 2, Bio-Rad Precision Plus Protein Dual-color, Thermo Fisher Spectra Multi-color. SeeBlue Plus2 Pre-Stained Standard contains 10 proteins (4250 kDa): 8 blue-dyed and 2 with contrasting colors, for easy and quick evaluation of electrophoresis and western transfer efficiency. Monitoring of protein transfer onto membranes during Western blots. The most widely used Western blotting protein standards are prestained proteins of known molecular mass (kDa). Monitoring of protein migration during SDS-PAGE. Ready-to-use: supplied in a loading buffer for direct loading on gelsĮasy to identify: includes the ~25, ~75 kDa reference bands coupled with a green and a red dye Do not heats, dilute, and add reducing agent before loading.īroad range: 3.5-245 kDa (Tris-glycine-SDS running buffer)
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Learn how to prepare, load, and store the standard, as well as troubleshoot common issues. The ladder is supplied in gel loading buffer and is ready to use. This manual provides detailed instructions on how to use the SeeBlue Plus2 Pre-stained Protein Standard, a ready-to-use mixture of 10 proteins with different molecular weights and colors for electrophoresis and Western blotting applications. The SeeBlue Plus2 Pre-Stained Standard contains 10 proteins: 8 blue-dyed and 2 with contrasting colors, providing easier band identification. The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-PAGE, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of p roteins. Towbin et al in 1979 ( Towbin, Staehelin, & Gordon. The protein standard is supplied in a ready-to-use format for direct. The standards consist of three blue-dyed and two colored bands ranging in size from 21261 kDa for monitoring gel and transfer runs. It is built on a technique that involves transferring, also known as blotting, proteins separated by electrophoresis from the gel to a membrane where they can be visualized specifically. E-PAGE SeeBlue Pre-stained Standards are designed for use with the pre-cast E-PAGE Electrophoresis System. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with Tris-glycine-SDS running buffer. Western Blot (WB) is a common method to detect and analyze proteins. The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 prestained proteins covering a wide range molecular weights from 3.5 to 245 kilodalton (kDa).